{"id":5525,"date":"2016-09-08T06:28:11","date_gmt":"2016-09-08T11:28:11","guid":{"rendered":"https:\/\/www.kabt.org\/?p=5525"},"modified":"2016-09-07T21:07:57","modified_gmt":"2016-09-08T02:07:57","slug":"tbt-spinach-chloroplasts","status":"publish","type":"post","link":"https:\/\/www.kabt.org\/?p=5525","title":{"rendered":"TBT: Spinach Chloroplasts"},"content":{"rendered":"

Editor’s Note: This post was originally published in September 2014 by our resident tinker, Michael Ralph<\/a>. I think he was successful in staining\u00a0chloroplasts, how about you?<\/strong><\/span><\/p>\n

I was pondering how to get a good look at plant cells with low cost, and I thought about Brad’s work with onion root tips in visualizing mitotic cells. Check out his original post here<\/a>. The thought occurred to me that the fixative should dissolve inter-cellular connections in leaf tissue the same as root tissue, so I gave a section of grocery store spinach tissue the same 6 minute warm fixative bath. The tissue flattened nicely (more or less), but I couldn’t see much in the way of cell definition. Sticking with the theme, I grabbed the aceto-orcein stain because it was already handy. Here’s what I saw:<\/p>\n

\"Spinach<\/a>

Spinach Cells – Aceto-orcein stain<\/p><\/div>\n

The remarkable definition in the organelle structure was surprising. Aceto-orcein binds DNA, so what would produce such well-defined structures that contain DNA. How about chloroplasts?<\/p>\n

\"Plagiomnium<\/a>

Plagiomnium ellipticum cells with visible chloroplasts.<\/p><\/div>\n

Let me know in the comments section: \u00a0chloroplasts or not? Alternative explanations?<\/p>\n","protected":false},"excerpt":{"rendered":"

Editor’s Note: This post was originally published in September 2014 by our resident tinker, Michael Ralph. I think he was successful in staining\u00a0chloroplasts, how about you? I was pondering how to get a good look at plant cells with low cost, and I thought about Brad’s work with onion root tips in visualizing mitotic cells.
+ Read More<\/a><\/p>\n","protected":false},"author":5666,"featured_media":4741,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_s2mail":"yes","_uf_show_specific_survey":0,"_uf_disable_surveys":false,"footnotes":""},"categories":[38,651],"tags":[664,666,665,652],"class_list":["post-5525","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-student-research-ideas","category-tbt","tag-chloroplasts","tag-microscopy","tag-staining","tag-tbt"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.kabt.org\/index.php?rest_route=\/wp\/v2\/posts\/5525","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.kabt.org\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.kabt.org\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.kabt.org\/index.php?rest_route=\/wp\/v2\/users\/5666"}],"replies":[{"embeddable":true,"href":"https:\/\/www.kabt.org\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=5525"}],"version-history":[{"count":2,"href":"https:\/\/www.kabt.org\/index.php?rest_route=\/wp\/v2\/posts\/5525\/revisions"}],"predecessor-version":[{"id":5527,"href":"https:\/\/www.kabt.org\/index.php?rest_route=\/wp\/v2\/posts\/5525\/revisions\/5527"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.kabt.org\/index.php?rest_route=\/wp\/v2\/media\/4741"}],"wp:attachment":[{"href":"https:\/\/www.kabt.org\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=5525"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.kabt.org\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=5525"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.kabt.org\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=5525"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}